Development and Evaluation of a New Lateral Flow Immunoassay for Serodiagnosis of Human Fasciolosis

نویسندگان

  • Victoria Martínez-Sernández
  • Laura Muiño
  • María Jesús Perteguer
  • Teresa Gárate
  • Mercedes Mezo
  • Marta González-Warleta
  • Antonio Muro
  • José Manuel Correia da Costa
  • Fernanda Romarís
  • Florencio M. Ubeira
چکیده

BACKGROUND Human fasciolosis is a re-emerging disease worldwide and is caused by species of the genus Fasciola (F. hepatica and F. gigantica). Human fasciolosis can be diagnosed by classical coprological techniques, such as the Kato-Katz test, to reveal parasite eggs in faeces. However, although 100% specific, these methods are generally not adequate for detection of acute infections, ectopic infections, or infections with low number of parasites. In such cases immunological methods may be a good alternative and are recommended for use in major hospitals where trained personnel are available, although they are not usually implemented for individual testing. METHODOLOGY/PRINCIPAL FINDINGS We have developed a new lateral flow test (SeroFluke) for the serodiagnosis of human fasciolosis. The new test was constructed with a recombinant cathepsin L1 from F. hepatica, and uses protein A and mAb MM3 as detector reagents in the test and control lines, respectively. In comparison with an ELISA test (MM3-SERO) the SeroFluke test showed maximal specificity and sensitivity and can be used with serum or whole blood samples. CONCLUSIONS/SIGNIFICANCE The new test can be used in major hospitals in hypoendemic countries as well as in endemic/hyperendemic regions where point-of-care testing is required.

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عنوان ژورنال:

دوره 5  شماره 

صفحات  -

تاریخ انتشار 2011